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It functions by blocking an enzyme known as nicotinamide N-methyltransferase (NNMT), which is important for how the body stores fat and uses energy

Mechanism of Action in Research Models AOD-9604 is researched for its involvement in lipolysis pathways, the process of breaking down stored fat for energy.Because it retains only the C-terminal fragment of the hGH molecule, researchers use it to isolate this specific activity from the broader hormonal cascade triggered by full hGH, allowing for more targeted study of fat-metabolism mechanisms without the confounding variables introduced by growth-hormone-axis activation

Also, it acts as a lipotropic agent to prevent fat build up in the liver and body

In vitro studies confirm minimal cytotoxicity with no impact on cell viability up to 100 M concentrations across multiple cell types, though higher concentrations may produce non-specific effects that could confound experimental results